Journal: EMBO Reports

Article Title: Muskelin is a substrate adaptor of the highly regulated Drosophila embryonic CTLH E3 ligase

doi: 10.1038/s44319-025-00397-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-RanBPM , Boster Biological Technology , DZ41192.

Techniques: SYBR Green Assay, Imaging, Recombinant, Sequencing, Software, Microscopy, Ion-Mobility Spectrometry, Mass Spectrometry

Journal: EMBO Reports

Article Title: Muskelin is a substrate adaptor of the highly regulated Drosophila embryonic CTLH E3 ligase

doi: 10.1038/s44319-025-00397-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-RanBPM , Boster Biological Technology , DZ41192.

Techniques: SYBR Green Assay, Imaging, Recombinant, Sequencing, Software, Microscopy, Ion-Mobility Spectrometry, Mass Spectrometry

Journal: Cellular oncology (Dordrecht)

Article Title: ATM-Mediated translocation of RanBPM regulates DNA damage response by stabilizing p21 in non-small cell lung cancer cells.

doi: 10.1007/s13402-023-00866-x

Figure Lengend Snippet: Fig. 7 DNA damage promotes the translocation of RanBPM and regulates p21 protein stability through ATM-mediated pathways. (A-C) A549 or H1299 cells were treated with 5μM Etop or 0.5μM Dox in the presence or absence of ATM inhibitor KU-55,933 (10μM), and the total proteins were assessed by west ern blotting. After cell fraction ation, the subcellular fractions were blotted with the indicated antibodies(C). (D and E) A549 or H1299 cells were treated with 5μM Etop or 0.5μM Dox in the presence or absence of ATM inhibitor KU-55,933 (10μM), and the cells were stained with the indicated fluorescent antibodies. DAPI was used for nuclei stain ing. Scale bars, 20 μm

Article Snippet: Specifically, incubation with antibodies against RanBPM (1:50 dilution; Santa Cruz, USA), p21 (1:100 dilution; CST) was carried out overnight at room temperature.

Techniques: Translocation Assay, Staining

Journal: Cellular oncology (Dordrecht)

Article Title: ATM-Mediated translocation of RanBPM regulates DNA damage response by stabilizing p21 in non-small cell lung cancer cells.

doi: 10.1007/s13402-023-00866-x

Figure Lengend Snippet: Fig. 8 Schematic representation of p21 regulated by RanBPM-USP11 axis

Article Snippet: Specifically, incubation with antibodies against RanBPM (1:50 dilution; Santa Cruz, USA), p21 (1:100 dilution; CST) was carried out overnight at room temperature.

Techniques:

Journal: Cellular oncology (Dordrecht)

Article Title: ATM-Mediated translocation of RanBPM regulates DNA damage response by stabilizing p21 in non-small cell lung cancer cells.

doi: 10.1007/s13402-023-00866-x

Figure Lengend Snippet: Fig. 7 DNA damage promotes the translocation of RanBPM and regulates p21 protein stability through ATM-mediated pathways. (A-C) A549 or H1299 cells were treated with 5μM Etop or 0.5μM Dox in the presence or absence of ATM inhibitor KU-55,933 (10μM), and the total proteins were assessed by west ern blotting. After cell fraction ation, the subcellular fractions were blotted with the indicated antibodies(C). (D and E) A549 or H1299 cells were treated with 5μM Etop or 0.5μM Dox in the presence or absence of ATM inhibitor KU-55,933 (10μM), and the cells were stained with the indicated fluorescent antibodies. DAPI was used for nuclei stain ing. Scale bars, 20 μm

Article Snippet: Specific antibodies against p53 (DO-1, sc-126), p15 (D-12, sc-271,791), p19 (SPM429, sc-65,594) and RanBPM (sc271,727) antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA), and p18 (#2896), p21 (#2947), p57 (#2557) and RanBPM (#14,638) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Translocation Assay, Staining

Journal: Cellular oncology (Dordrecht)

Article Title: ATM-Mediated translocation of RanBPM regulates DNA damage response by stabilizing p21 in non-small cell lung cancer cells.

doi: 10.1007/s13402-023-00866-x

Figure Lengend Snippet: Fig. 8 Schematic representation of p21 regulated by RanBPM-USP11 axis

Article Snippet: Specific antibodies against p53 (DO-1, sc-126), p15 (D-12, sc-271,791), p19 (SPM429, sc-65,594) and RanBPM (sc271,727) antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA), and p18 (#2896), p21 (#2947), p57 (#2557) and RanBPM (#14,638) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: